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| Bolige, Aoen; Hagiwara, Shin-ya; Zhang, Yulan; Goto, Ken; 後藤, 健. |
| Cell population growth is gated to occur in particular circadian phases, which has been known for over four decades in various organisms including cyanobacteria and human. However, little is known as to which cell cycle phases from G(1) to M are primarily regulated by the circadian rhythm or when in a circadian cycle this primary regulation takes place. We report here that in the flagellate alga Euglena gracilis grown photoautotrophically, the circadian rhythm primarily prevented developmentally matured G(2) cells from progressing to mitosis, such that cell population growth occurred only during subjective night. In addition, we found that the circadian rhythm also arrests G(1)-to-S and S-to-G(2) transitions at particular circadian phases. |
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Palavras-chave: Cell cycle; Circadian rhythms; Englena gracilis; G2-arrest; Mitosis. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/717 |
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| Liu,Donghua; Jiang,Wusheng; Meng,Qingmin; Zou,Jin; Gu,Jiegang; Zeng,Muai. |
| Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. Also, increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Garlic; Organelle; Cytogenetical and ultrastructural alterations; Mitosis. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100003 |
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| Hernández-Quintero,JD; Reyes-Valdés,MH; Mendoza-Rodríguez,DV; Gómez-Martínez,M; Rodríguez-Herrera,R. |
| El género Dasylirion es un grupo de plantas típicas del Desierto Chihuahuense, perennes, de un comportamiento sexual dioico y denominadas comúnmente sotoles. Es un género poco estudiado desde el punto de vista biológico y se desconocen las bases de su dinámica reproductiva. En este trabajo se estudió el número cromosómico y el comportamiento meiótico de Dasylirion cedrosanum en el municipio de Saltillo, Coahuila, una localidad del noreste de México. Para la preparación de cromosomas mitóticos se utilizó una técnica basada en un tratamiento enzimático con pectoliasa y celulasa, así como tinción con acetocarmín. Para el estudio meiótico se recolectaron botones florales masculinos, se fijaron y se tiñeron con el mismo colorante. Como resultado, se reportan... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dasylirion cedrosanum; Dioecia; Cromosomas; Mitosis; Meiosis. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000100015 |
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| ANDRADA,Aldo Rubén; SILENZI-USANDIVARAS,Gabriela Mana; RUIZ,Ana Inés; PÁEZ,Valeria de los Angeles; ALBORNOZ,Patricia Liliana. |
| RESUMEN Talinum paniculatum es una hierba adventicia ampliamente distribuida en Argentina, que tiene importancia económica como maleza de cultivos resistente a herbicidas. Esta especie se presenta en el campo con dos morfotipos y ellos se distinguen por la forma, tamaño y color de sus flores, frutos y hojas. El objetivo de este trabajo fue determinar las características morfo-anatómicas (de hoja y tallo), citogenéticas y moleculares en dos morfotipos de la provincia de Tucumán (Argentina), y establecer diferencias entre ellos. Se utilizaron técnicas morfo-anatómicas y citogenéticas clásicas y se realizaron análisis moleculares con el marcador ITS2. Los resultados evidencian que las características morfológicas, anatómicas, citogenéticas y moleculares de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anatomía; Cromosomas; Filogenia; ITS2; Mitosis; Morfotipos. |
| Ano: 2020 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000300310 |
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| Joubert,Annie; Marais,Sumari; Maritz,Christine. |
| Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Breast carcinoma; Mitosis; Apoptosis. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100007 |
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| PIEROZZI,NEIVA IZABEL; MOURA,MARA FERNANDES. |
| ABSTRACT Seven species of Vitis L., V. champinii Planchon, V. cinerea (Engelm in Gray) Engelm, V. girdiana Munson, V. labrusca L., V. rotundifolia Michaux, V. rupestres Scheels and V. vinifera L. were studied with the purpose of complementing the karyomorphometric information for further comparative analyses. Based on ideograms and on chromosome measures obtained it was possible to see several differences among the species, which were enough to distinguish at least V. champinii and V. girdiana from the others as well as V. labrusca for the lowest measures and V. rotundifolia for the highest mean value of arm ratio. It seems that during the species diversification process the most crucial differences among them did not involve drastic changes in chromosome... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; Ideogram; Vitaceae; Mitosis; Satellite segment. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000100213 |
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| Uz,Gulsen; Sarikaya,Aysegul Topal. |
| Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459 |
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